Bioremediation of Diesel Oil Spill by Filamentous Fungus Trichoderma reesei H002 in Aquatic Environment
Keywords:
Oil spill treatment, Trichoderma reesei H002, bioremediation, TPHsAbstract
Bioremediation of aquatic environment could be a response to the oil spills threats. In this paper, Trichoderma reesei H002, a filamentous ascomycete fungus isolated from a polluted site in an orchard garden, Johor, Malaysia, was experimented for its biodegradation ability to degrade diesel oil. Varying nitrogen and carbon sources, pH, agitation on diesel oil by Trichoderma reesei H002 in liquid media were examined to find their impacts on TPHs, alkane, aromatic and NSO fractions of diesel oil degradation. Glucose and yeast extract were the most suitable nutrients for the development of T. reesei H002 and increased the degradation of total petroleum hydrocarbons (TPHs up to 94.78% at the end of the study (40 days) at 25 0C. The degradation of TPHs were performed by gravimetric analysis and degradation of alkane and aromatic fractions were confirmed by GC-FID analysis. Based on the findings of T. reesei H002 for the biodegradation of diesel oil, it can be proposed that T. reesei H002 can be applied to bioremediate diesel oil spill in aquatic environment, therefore protect the ecosystem.Downloads
Download data is not yet available.
Downloads
Published
01-01-2019
Issue
Section
Articles
License
Open access licenses
Open Access is by licensing the content with a Creative Commons (CC) license.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
How to Cite
Nazifa, T. H., Ahmad, M. A., Hadibarata, T., & Aris, A. (2019). Bioremediation of Diesel Oil Spill by Filamentous Fungus Trichoderma reesei H002 in Aquatic Environment. International Journal of Integrated Engineering, 10(9). https://penerbit.uthm.edu.my/ojs/index.php/ijie/article/view/2369