DNA Barcoding of Commercial Fish Sold in Muar Fish Market, Johor

Authors

  • Nursyuhada Othman Universiti Tun Hussein Onn Malaysia
  • Jaevilynecrisse Abel Universiti Tun Hussein Onn Malaysia
  • Hidayah Haris Universiti Tun Hussein Onn Malaysia
  • Mohd Lokman Ilham-Norhakim Universiti Tun Hussein Onn Malaysia
  • Kaviarasu Munian Universiti Tun Hussein Onn Malaysia
  • Farah Farhana Ramli Universiti Tun Hussein Onn Malaysia
  • Nur Hartini Sariyati Universiti Tun Hussein Onn Malaysia
  • Mohd Faudzir Najmuddin Universiti Tun Hussein Onn Malaysia
  • Muhammad Abu Bakar Abdul-Latiff Universiti Tun Hussein Onn Malaysia

Keywords:

Commercial Fish, fish market, DNA barcoding, fisheries, Muar

Abstract

Due to the high demand in the fisheries market, species identification is vital for commercial fish to avoid mislabeling and fraud in marketplaces. The increasingly threatening human activities like overfishing, blast fishing, poison fishing, and trawling have become the major threats to fish and caused an urgent need for documentation of both marine and freshwater fishes. Commercial fish products sometimes are sold by their parts, such as fins which further create confusion for identification purposes. Morphological identification also requires highly skilled ichthyologists to avoid misidentification. Thus, identification through DNA barcoding can be utilized as it holds the potential for accurate and rapid identification. Here we identified the commercial marine fish species sold in Muar Fish Market, Johor, using the Cytochrome Oxidase subunit I (COI) gene of mitochondrial DNA (mtDNA). A 700 base-pair sequence of the COI region was targeted by amplifying extracted DNA from 28 fin samples collected from the fish market. From 28 samples, we successfully sequenced 16 samples, and by using phylogenetic analysis, (Neighbor-Joining (NJ), Maximum Parsimony (MP) and Bayesian Inference (BI)) we successfully identified the samples belonging to nine families (Scombridae, Platycephalidae, Carangidae, Ariidae, Polynemidae, Coryphaenidae, Sphyraenidae, Muraenesocidae, Engraiulidae), 12 genera and 16 species. This study reports the first documentation of commercial fish sold in Muar through the DNA barcoding technique. This technique should be expanded further to identify any species with important conservation implications, such as endangered species, for improved management of fisheries in Malaysia.

Downloads

Published

08-02-2023

How to Cite

Othman, N. ., Abel, J. ., Haris, H. ., Ilham-Norhakim, M. L. ., Munian, K. ., Ramli, F. F., Sariyati, N. H., Najmuddin, M. F. ., & Abdul-Latiff, M. A. B. . (2023). DNA Barcoding of Commercial Fish Sold in Muar Fish Market, Johor. Journal of Sustainable Natural Resources, 3(2), 55–61. Retrieved from https://penerbit.uthm.edu.my/ojs/index.php/jsunr/article/view/13239

Issue

Section

Articles

Most read articles by the same author(s)